antibody absorption Search Results


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Biorbyt absorptive enterocytes
The number of proliferating cells in anterior intestine in embryonically-exposed killifish. Immunohistochemistry was used to examine the number of proliferating cells as measured by nuclear staining of PCNA (A; indicated by arrows), the number of goblet cells (B; indicated by arrows), and % <t>enterocytes</t> or <t>absorptive</t> surface (B) (representative images are shown of n = 6 fish per exposure group per time point). The number of PCNA+ cells were counted per 2 intervillus regions at a 40X field of view, and averaged per villus (C). Absorptive surface area was quantified by outlining 4 villi per field of view at 10X for absorptive surface and determining % stained cells using ImageJ (D). Goblet cells were counted from 4 villi per field of view at 10X, and averaged per villus (E). The colors were converted to a black and white image so only cytoplasmic staining of the enterocytes would be determined. All sections were averaged per group, and statistical significance (*) was determined by ANOVA followed by Tukey’s (p < 0.05).
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ZEUS Scientific fluorescent treponemal antibody absorption testing glass slides
The number of proliferating cells in anterior intestine in embryonically-exposed killifish. Immunohistochemistry was used to examine the number of proliferating cells as measured by nuclear staining of PCNA (A; indicated by arrows), the number of goblet cells (B; indicated by arrows), and % <t>enterocytes</t> or <t>absorptive</t> surface (B) (representative images are shown of n = 6 fish per exposure group per time point). The number of PCNA+ cells were counted per 2 intervillus regions at a 40X field of view, and averaged per villus (C). Absorptive surface area was quantified by outlining 4 villi per field of view at 10X for absorptive surface and determining % stained cells using ImageJ (D). Goblet cells were counted from 4 villi per field of view at 10X, and averaged per villus (E). The colors were converted to a black and white image so only cytoplasmic staining of the enterocytes would be determined. All sections were averaged per group, and statistical significance (*) was determined by ANOVA followed by Tukey’s (p < 0.05).
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The number of proliferating cells in anterior intestine in embryonically-exposed killifish. Immunohistochemistry was used to examine the number of proliferating cells as measured by nuclear staining of PCNA (A; indicated by arrows), the number of goblet cells (B; indicated by arrows), and % <t>enterocytes</t> or <t>absorptive</t> surface (B) (representative images are shown of n = 6 fish per exposure group per time point). The number of PCNA+ cells were counted per 2 intervillus regions at a 40X field of view, and averaged per villus (C). Absorptive surface area was quantified by outlining 4 villi per field of view at 10X for absorptive surface and determining % stained cells using ImageJ (D). Goblet cells were counted from 4 villi per field of view at 10X, and averaged per villus (E). The colors were converted to a black and white image so only cytoplasmic staining of the enterocytes would be determined. All sections were averaged per group, and statistical significance (*) was determined by ANOVA followed by Tukey’s (p < 0.05).
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Mast Diagnostica GmbH fluorescent treponemal antibody absorption test fta-abs
The number of proliferating cells in anterior intestine in embryonically-exposed killifish. Immunohistochemistry was used to examine the number of proliferating cells as measured by nuclear staining of PCNA (A; indicated by arrows), the number of goblet cells (B; indicated by arrows), and % <t>enterocytes</t> or <t>absorptive</t> surface (B) (representative images are shown of n = 6 fish per exposure group per time point). The number of PCNA+ cells were counted per 2 intervillus regions at a 40X field of view, and averaged per villus (C). Absorptive surface area was quantified by outlining 4 villi per field of view at 10X for absorptive surface and determining % stained cells using ImageJ (D). Goblet cells were counted from 4 villi per field of view at 10X, and averaged per villus (E). The colors were converted to a black and white image so only cytoplasmic staining of the enterocytes would be determined. All sections were averaged per group, and statistical significance (*) was determined by ANOVA followed by Tukey’s (p < 0.05).
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EUROIMMUN fluorescent treponemal antibody absorption (fta-abs) t. pallidum -igg
The number of proliferating cells in anterior intestine in embryonically-exposed killifish. Immunohistochemistry was used to examine the number of proliferating cells as measured by nuclear staining of PCNA (A; indicated by arrows), the number of goblet cells (B; indicated by arrows), and % <t>enterocytes</t> or <t>absorptive</t> surface (B) (representative images are shown of n = 6 fish per exposure group per time point). The number of PCNA+ cells were counted per 2 intervillus regions at a 40X field of view, and averaged per villus (C). Absorptive surface area was quantified by outlining 4 villi per field of view at 10X for absorptive surface and determining % stained cells using ImageJ (D). Goblet cells were counted from 4 villi per field of view at 10X, and averaged per villus (E). The colors were converted to a black and white image so only cytoplasmic staining of the enterocytes would be determined. All sections were averaged per group, and statistical significance (*) was determined by ANOVA followed by Tukey’s (p < 0.05).
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Trinity Biotech fluorescent treponemal antibody-absorption test (fta-abs)
The number of proliferating cells in anterior intestine in embryonically-exposed killifish. Immunohistochemistry was used to examine the number of proliferating cells as measured by nuclear staining of PCNA (A; indicated by arrows), the number of goblet cells (B; indicated by arrows), and % <t>enterocytes</t> or <t>absorptive</t> surface (B) (representative images are shown of n = 6 fish per exposure group per time point). The number of PCNA+ cells were counted per 2 intervillus regions at a 40X field of view, and averaged per villus (C). Absorptive surface area was quantified by outlining 4 villi per field of view at 10X for absorptive surface and determining % stained cells using ImageJ (D). Goblet cells were counted from 4 villi per field of view at 10X, and averaged per villus (E). The colors were converted to a black and white image so only cytoplasmic staining of the enterocytes would be determined. All sections were averaged per group, and statistical significance (*) was determined by ANOVA followed by Tukey’s (p < 0.05).
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Becton Dickinson fluorescent treponemal antibody absorption (fta-abs) test
The number of proliferating cells in anterior intestine in embryonically-exposed killifish. Immunohistochemistry was used to examine the number of proliferating cells as measured by nuclear staining of PCNA (A; indicated by arrows), the number of goblet cells (B; indicated by arrows), and % <t>enterocytes</t> or <t>absorptive</t> surface (B) (representative images are shown of n = 6 fish per exposure group per time point). The number of PCNA+ cells were counted per 2 intervillus regions at a 40X field of view, and averaged per villus (C). Absorptive surface area was quantified by outlining 4 villi per field of view at 10X for absorptive surface and determining % stained cells using ImageJ (D). Goblet cells were counted from 4 villi per field of view at 10X, and averaged per villus (E). The colors were converted to a black and white image so only cytoplasmic staining of the enterocytes would be determined. All sections were averaged per group, and statistical significance (*) was determined by ANOVA followed by Tukey’s (p < 0.05).
Fluorescent Treponemal Antibody Absorption (Fta Abs) Test, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Reagen LLC fluorescent treponemal antibody absorption test (fta-abs)
The number of proliferating cells in anterior intestine in embryonically-exposed killifish. Immunohistochemistry was used to examine the number of proliferating cells as measured by nuclear staining of PCNA (A; indicated by arrows), the number of goblet cells (B; indicated by arrows), and % <t>enterocytes</t> or <t>absorptive</t> surface (B) (representative images are shown of n = 6 fish per exposure group per time point). The number of PCNA+ cells were counted per 2 intervillus regions at a 40X field of view, and averaged per villus (C). Absorptive surface area was quantified by outlining 4 villi per field of view at 10X for absorptive surface and determining % stained cells using ImageJ (D). Goblet cells were counted from 4 villi per field of view at 10X, and averaged per villus (E). The colors were converted to a black and white image so only cytoplasmic staining of the enterocytes would be determined. All sections were averaged per group, and statistical significance (*) was determined by ANOVA followed by Tukey’s (p < 0.05).
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Johns Hopkins HealthCare fluorescent treponemal antibody absorption test
The number of proliferating cells in anterior intestine in embryonically-exposed killifish. Immunohistochemistry was used to examine the number of proliferating cells as measured by nuclear staining of PCNA (A; indicated by arrows), the number of goblet cells (B; indicated by arrows), and % <t>enterocytes</t> or <t>absorptive</t> surface (B) (representative images are shown of n = 6 fish per exposure group per time point). The number of PCNA+ cells were counted per 2 intervillus regions at a 40X field of view, and averaged per villus (C). Absorptive surface area was quantified by outlining 4 villi per field of view at 10X for absorptive surface and determining % stained cells using ImageJ (D). Goblet cells were counted from 4 villi per field of view at 10X, and averaged per villus (E). The colors were converted to a black and white image so only cytoplasmic staining of the enterocytes would be determined. All sections were averaged per group, and statistical significance (*) was determined by ANOVA followed by Tukey’s (p < 0.05).
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Scimedx Inc fluorescent treponemal antibody absorption test igm
The number of proliferating cells in anterior intestine in embryonically-exposed killifish. Immunohistochemistry was used to examine the number of proliferating cells as measured by nuclear staining of PCNA (A; indicated by arrows), the number of goblet cells (B; indicated by arrows), and % <t>enterocytes</t> or <t>absorptive</t> surface (B) (representative images are shown of n = 6 fish per exposure group per time point). The number of PCNA+ cells were counted per 2 intervillus regions at a 40X field of view, and averaged per villus (C). Absorptive surface area was quantified by outlining 4 villi per field of view at 10X for absorptive surface and determining % stained cells using ImageJ (D). Goblet cells were counted from 4 villi per field of view at 10X, and averaged per villus (E). The colors were converted to a black and white image so only cytoplasmic staining of the enterocytes would be determined. All sections were averaged per group, and statistical significance (*) was determined by ANOVA followed by Tukey’s (p < 0.05).
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Microgen Bioproducts im absorption test for heterphil antibody
The number of proliferating cells in anterior intestine in embryonically-exposed killifish. Immunohistochemistry was used to examine the number of proliferating cells as measured by nuclear staining of PCNA (A; indicated by arrows), the number of goblet cells (B; indicated by arrows), and % <t>enterocytes</t> or <t>absorptive</t> surface (B) (representative images are shown of n = 6 fish per exposure group per time point). The number of PCNA+ cells were counted per 2 intervillus regions at a 40X field of view, and averaged per villus (C). Absorptive surface area was quantified by outlining 4 villi per field of view at 10X for absorptive surface and determining % stained cells using ImageJ (D). Goblet cells were counted from 4 villi per field of view at 10X, and averaged per villus (E). The colors were converted to a black and white image so only cytoplasmic staining of the enterocytes would be determined. All sections were averaged per group, and statistical significance (*) was determined by ANOVA followed by Tukey’s (p < 0.05).
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Shipped at 4°C. Upon delivery aliquot and store at-20°C or-80°C. Avoid repeated freeze / thaw cycles.http://www.creative-diagnostics.com/Anti-Zebrafish-Gut-Absorptive-Cell-Epitopes-Antibody-130546-144.htm
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The number of proliferating cells in anterior intestine in embryonically-exposed killifish. Immunohistochemistry was used to examine the number of proliferating cells as measured by nuclear staining of PCNA (A; indicated by arrows), the number of goblet cells (B; indicated by arrows), and % enterocytes or absorptive surface (B) (representative images are shown of n = 6 fish per exposure group per time point). The number of PCNA+ cells were counted per 2 intervillus regions at a 40X field of view, and averaged per villus (C). Absorptive surface area was quantified by outlining 4 villi per field of view at 10X for absorptive surface and determining % stained cells using ImageJ (D). Goblet cells were counted from 4 villi per field of view at 10X, and averaged per villus (E). The colors were converted to a black and white image so only cytoplasmic staining of the enterocytes would be determined. All sections were averaged per group, and statistical significance (*) was determined by ANOVA followed by Tukey’s (p < 0.05).

Journal: Journal of toxicology and environmental health. Part A

Article Title: Embryonic Arsenic Exposure Reduces Intestinal Cell Proliferation and Alters Hepatic IGF mRNA Expression in Killifish ( Fundulus heteroclitus )

doi: 10.1080/15287394.2019.1571465

Figure Lengend Snippet: The number of proliferating cells in anterior intestine in embryonically-exposed killifish. Immunohistochemistry was used to examine the number of proliferating cells as measured by nuclear staining of PCNA (A; indicated by arrows), the number of goblet cells (B; indicated by arrows), and % enterocytes or absorptive surface (B) (representative images are shown of n = 6 fish per exposure group per time point). The number of PCNA+ cells were counted per 2 intervillus regions at a 40X field of view, and averaged per villus (C). Absorptive surface area was quantified by outlining 4 villi per field of view at 10X for absorptive surface and determining % stained cells using ImageJ (D). Goblet cells were counted from 4 villi per field of view at 10X, and averaged per villus (E). The colors were converted to a black and white image so only cytoplasmic staining of the enterocytes would be determined. All sections were averaged per group, and statistical significance (*) was determined by ANOVA followed by Tukey’s (p < 0.05).

Article Snippet: Paraffin embedded intestinal sections (7 μm) were examined for markers of absorptive enterocytes (gut absorptive cell epitope antibody; Biorbyt, # orb324078), while the number of proliferating cells was determined by expression of proliferating cell nuclear antigen (PCNA) (Abcam, #ab29).

Techniques: Immunohistochemistry, Staining