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Image Search Results
Journal: Journal of toxicology and environmental health. Part A
Article Title: Embryonic Arsenic Exposure Reduces Intestinal Cell Proliferation and Alters Hepatic IGF mRNA Expression in Killifish ( Fundulus heteroclitus )
doi: 10.1080/15287394.2019.1571465
Figure Lengend Snippet: The number of proliferating cells in anterior intestine in embryonically-exposed killifish. Immunohistochemistry was used to examine the number of proliferating cells as measured by nuclear staining of PCNA (A; indicated by arrows), the number of goblet cells (B; indicated by arrows), and % enterocytes or absorptive surface (B) (representative images are shown of n = 6 fish per exposure group per time point). The number of PCNA+ cells were counted per 2 intervillus regions at a 40X field of view, and averaged per villus (C). Absorptive surface area was quantified by outlining 4 villi per field of view at 10X for absorptive surface and determining % stained cells using ImageJ (D). Goblet cells were counted from 4 villi per field of view at 10X, and averaged per villus (E). The colors were converted to a black and white image so only cytoplasmic staining of the enterocytes would be determined. All sections were averaged per group, and statistical significance (*) was determined by ANOVA followed by Tukey’s (p < 0.05).
Article Snippet: Paraffin embedded intestinal sections (7 μm) were examined for markers of
Techniques: Immunohistochemistry, Staining